Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 15, 2010
Previous Article Next Article

Image correlation spectroscopy as a tool for microrheology of soft materials

Author affiliations

Abstract

Image correlation spectroscopy (ICS) has been mostly used to quantify spatiotemporal events in biological experiments. Here, we develop and demonstrate the use of ICS for microrheology (μR) of complex, viscoelastic materials. A special technique for extracting mean-squared displacements (MSD) of probe particles in the samples from image correlation data is developed. The method (ICS-μR) is tested on Newtonian (glycerol solutions in water) and complex fluids (poly(ethylene oxide) (PEO) solutions). Accurate measurements of the viscosities of the Newtonian fluids (equivalently, the diffusion coefficients of the probe particles) as well as the storage and loss moduli of the PEO solutions could be obtained. The possibility to combine spatiotemporal assessment and time- and length-scale dependent microrheological measurements from images of fluorescent molecules makes ICS-μR a prospective tool in many biophysical applications. Further, the method developed for extracting MSD from image (or intensity) correlation measurements can be applied to other microrheological techniques to provide improved accuracy of rheological measurements.

Graphical abstract: Image correlation spectroscopy as a tool for microrheology of soft materials

Back to tab navigation

Supplementary files

Article information


Submitted
02 Feb 2010
Accepted
19 Apr 2010
First published
10 Jun 2010

Soft Matter, 2010,6, 3499-3505
Article type
Paper

Image correlation spectroscopy as a tool for microrheology of soft materials

N. A. Kurniawan, C. T. Lim and R. Rajagopalan, Soft Matter, 2010, 6, 3499
DOI: 10.1039/C002265A

Social activity

Search articles by author

Spotlight

Advertisements