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Issue 10, 2010
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Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore

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Abstract

Aiming at the rational development of genetically-encoded photosensitisers, the production of singlet oxygen has been assessed for a number of class-2 Green Fluorescent Protein (GFP) mutants by means of time-resolved near-infrared luminescence detection. The accessibility of molecular oxygen to the chromophore seems to play a major role in the ability of GFPs to photosensitise singlet oxygen and this can be modulated by introducing specific mutations such as replacement of His148 by a less bulky amino acid. GFPs are also good singlet oxygen quenchers, hence further developments in this area should also seek to eliminate those amino acids with the highest quenching ability, particularly those at the protein surface and in the vicinity of the chromophore.

Graphical abstract: Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore

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Article information


Submitted
31 May 2010
Accepted
30 Jun 2010
First published
30 Jul 2010

Photochem. Photobiol. Sci., 2010,9, 1336-1341
Article type
Paper

Singlet oxygen photosensitisation by GFP mutants: oxygen accessibility to the chromophore

A. Jiménez-Banzo, X. Ragàs, S. Abbruzzetti, C. Viappiani, B. Campanini, C. Flors and S. Nonell, Photochem. Photobiol. Sci., 2010, 9, 1336
DOI: 10.1039/C0PP00125B

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