Jump to main content
Jump to site search

Issue 5, 2010
Previous Article Next Article

Preparation, characterization and application of polyaniline nanospheres to biosensing

Author affiliations

Abstract

Polyaniline nanospheres (PANI-NS) prepared by morphological transformation of micelle polymerized camphorsulfonic acid (CSA) doped polyaniline nanotubes (PANI-NT) in the presence of ethylene glycol (EG) have been characterized by X-ray diffraction, atomic force microscopy, transmission electron microscopy, scanning electron microscopy, Fourier transform infra-red and UV-Visible spectroscopy. A PANI-NS (60–80 nm) film deposited onto an indium-tin-oxide (ITO) coated glass plate by the solution casting method has been utilized for covalent immobilization of biomolecules (cholesterol oxidase (ChOx)) viaN-ethyl-N′-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry for fabrication of a cholesterol biosensor. The ChOx/PANI-NS/ITO bioelectrode detects cholesterol in the concentration range of 25 to 500 mg dL−1 with sensitivity of 1.3 × 10−3 mA mg−1 dL and regression coefficient of 0.98. Further, this PANI-NS based bioelectrode shows fast response time (10 s), low Michaelis–Menten constant (2.5 mM) and shelf-life of 12 weeks. The spherical nanostructure observed in the final morphology of the PANI-NS film is attributed to hydrogen bonding interactions between PANI-NT and EG.

Graphical abstract: Preparation, characterization and application of polyaniline nanospheres to biosensing

Back to tab navigation

Article information


Submitted
10 Nov 2009
Accepted
18 Jan 2010
First published
09 Mar 2010

Nanoscale, 2010,2, 747-754
Article type
Paper

Preparation, characterization and application of polyaniline nanospheres to biosensing

C. Dhand, M. Das, G. Sumana, A. K. Srivastava, M. K. Pandey, C. G. Kim, M. Datta and B. D. Malhotra, Nanoscale, 2010, 2, 747
DOI: 10.1039/B9NR00346K

Social activity

Search articles by author

Spotlight

Advertisements