Jump to main content
Jump to site search

Issue 9, 2010
Previous Article Next Article

One is enough: insights into the two-metal ion nuclease mechanism from global analysis and computational studies

Author affiliations

Abstract

The mechanistic details of metallonuclease reactions, typically supported by Mg(II), have a long and contentious history. Two-metal ion mechanisms have enjoyed much favor, based largely in the multitude of X-ray crystal structures of these enzymes with more than one metal ion per active site. Most recently, this mechanism has come under challenge. Reviewed herein are the applications of different experimental strategies that collectively support a mechanism in which only one metal ion is necessary for nucleic acid hydrolysis. Based on global kinetic analysis, analysis of reactions in which the nonsupportive Ca(II) is added, and a number of computational approaches, secondary sites are proposed to either be occupied by activity-modulating metal ions or occupied in turn by a single metal that changes position during the course of the reaction.

Graphical abstract: One is enough: insights into the two-metal ion nuclease mechanism from global analysis and computational studies

Back to tab navigation

Publication details

The article was received on 12 May 2010, accepted on 05 Jul 2010 and first published on 06 Aug 2010


Article type: Critical Review
DOI: 10.1039/C0MT00013B
Metallomics, 2010,2, 609-620

  •   Request permissions

    One is enough: insights into the two-metal ion nuclease mechanism from global analysis and computational studies

    C. M. Dupureur, Metallomics, 2010, 2, 609
    DOI: 10.1039/C0MT00013B

Search articles by author

Spotlight

Advertisements