Jump to main content
Jump to site search

Issue 21, 2010
Previous Article Next Article

Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

Author affiliations

Abstract

An agarose droplet method was developed for highly parallel and efficient single molecule emulsion PCR. The method capitalizes on the unique thermoresponsive sol–gel switching property of agarose for highly efficient DNA amplification and amplicon trapping. Uniform agarose solution droplets generated via a microfluidic chip serve as robust and inert nanolitre PCR reactors for single copy DNA molecule amplification. After PCR, agarose droplets are gelated to form agarose beads, trapping all amplicons in each reactor to maintain the monoclonality of each droplet. This method does not require cocapsulation of primer labeled microbeads, allows high throughput generation of uniform droplets and enables high PCR efficiency, making it a promising platform for many single copy genetic studies.

Graphical abstract: Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

Back to tab navigation

Supplementary files

Article information


Submitted
24 Jun 2010
Accepted
09 Aug 2010
First published
13 Sep 2010

Lab Chip, 2010,10, 2841-2843
Article type
Communication

Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

X. Leng, W. Zhang, C. Wang, L. Cui and C. J. Yang, Lab Chip, 2010, 10, 2841
DOI: 10.1039/C0LC00145G

Social activity

Search articles by author

Spotlight

Advertisements