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Issue 22, 2010
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Size-selective immunofluorescence of Mycobacterium tuberculosiscells by capillary- and viscous forces†

Woon-Hong Yeo,‡a   Fong-Li Chou,‡a   Gareth Fotouhi,a   Kieseok Oh,a   Blake T. Stevens,a   Hsiu-Yang Tseng,a   Dayong Gao,a   Amy Q. Shen,a   Jae-Hyun Chung*a  and  Kyong-Hoon Lee*b  
Author affiliations

* Corresponding authors

a Department of Mechanical Engineering, University of Washington, Seattle, WA, United States
E-mail: jae71@u.washington.edu
Fax: +1 206 685 8047
Tel: +1 206 543 4355

b NanoFacture, Inc., P.O. Box 52651, Bellevue, WA, United States
E-mail: hoonlee@nano-facture.com
Fax: +1 425 679 6207
Tel: +1 425 223 5723

Abstract

Rapid, low cost screening of tuberculosis requires an effective enrichment method of Mycobacterium tuberculosis (MTB) cells. Currently, microfiltration and centrifugation steps are frequently used for sample preparation, which are cumbersome and time-consuming. In this study, the size-selective capturing mechanism of a microtip-sensor is presented to directly enrich MTB cells from a sample mixture. When a microtip is withdrawn from a spherical suspension in the radial direction, the cells that are concentrated by AC electroosmosis are selectively enriched to the tip due to capillary- and viscous forces. The size-selectivity is characterized by using polystyrene microspheres, which is then applied to size-selective capture of MTB from a sample mixture. Our approach yields a detection limit of 800 cells mL−1, one of the highest-sensitivity immunosensors to date.

Graphical abstract: Size-selective immunofluorescence of Mycobacterium tuberculosis cells by capillary- and viscous forces

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Article information

https://doi.org/10.1039/C0LC00077A
Submitted
08 Jun 2010
Accepted
06 Aug 2010
First published
23 Sep 2010
Lab Chip, 2010,10, 3178-3181
Article type
Technical Note
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Size-selective immunofluorescence of Mycobacterium tuberculosis cells by capillary- and viscous forces

W. Yeo, F. Chou, G. Fotouhi, K. Oh, B. T. Stevens, H. Tseng, D. Gao, A. Q. Shen, J. Chung and K. Lee, Lab Chip, 2010, 10, 3178
DOI: 10.1039/C0LC00077A

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