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Issue 18, 2010
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A microfluidic platform for probing small artery structure and function

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Abstract

Although pathologic changes to the structure and function of small blood vessels are hallmarks of various cardiovascular diseases, limitations of conventional investigation methods (i.e. pressure myography) have prohibited a comprehensive understanding of the underlying mechanisms. We developed a microfluidic device to facilitate assessment of resistance artery structure and function under physiological conditions (37 °C, 45 mmHg transmural pressure). The platform allows for on-chip fixation, long-term culture and fully automated acquisition of up to ten dose–response sequences of intact mouse mesenteric artery segments (diameter ≈ 250 micrometres and length ≈ 1.5 mm) in a well-defined microenvironment. Even abluminal application of phenylephrine or acetylcholine (homogeneous condition) yielded dose–response relationships virtually identical to conventional myography. Unilateral application of phenylephrine (heterogeneous condition) limited constriction to the drug-exposed side, suggesting a lack of circumferential communication. The microfluidic platform allows us to address new fundamental biological questions, replaces a manually demanding procedure with a scalable approach and may enable organ-based screens to be routinely performed during drug development.

Graphical abstract: A microfluidic platform for probing small artery structure and function

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Article information


Submitted
25 Mar 2010
Accepted
14 Jun 2010
First published
06 Jul 2010

Lab Chip, 2010,10, 2341-2349
Article type
Paper

A microfluidic platform for probing small artery structure and function

A. Günther, S. Yasotharan, A. Vagaon, C. Lochovsky, S. Pinto, J. Yang, C. Lau, J. Voigtlaender-Bolz and S. Bolz, Lab Chip, 2010, 10, 2341
DOI: 10.1039/C004675B

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