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Issue 16, 2010
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ELISA-LOC: lab-on-a-chip for enzyme-linked immunodetection

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Abstract

A miniature 96 sample ELISA-lab-on-a-chip (ELISA-LOC) was designed, fabricated, and tested for immunological detection of Staphylococcal Enterotoxin B (SEB). The chip integrates a simple microfluidics system into a miniature ninety-six sample plate, allowing the user to carry out an immunological assay without a laboratory. Assay reagents are delivered into the assay plate without the need for separate devices commonly used in immunoassays. The ELISA-LOC was constructed using Laminated Object Manufacturing (LOM) technology to assemble six layers with an acrylic (poly(methyl methacrylate) (PMMA)) core and five polycarbonate layers micromachined by a CO2 laser. The ELISA-LOC has three main functional elements: reagent loading fluidics, assay and detection wells, and reagent removal fluidics, a simple “surface tension” valve used to control the flow. To enhance assay sensitivity and to perform the assay without a lab, ELISA-LOC detection combines several biosensing elements: (1) carbon nanotube (CNT) technology to enhance primary antibody immobilization, (2) sensitive ECL (electrochemiluminescence) detection, and (3) a charge-coupled device (CCD) detector for measuring the light signal generated by ECL. Using a sandwich ELISA assay, the system detected SEB at concentrations as low as 0.1 ng ml−1, which is similar to the reported sensitivity of conventional ELISA. The fluidics system can be operated by a syringe and does not require power for operation. This simple point-of-care (POC) system is useful for carrying out various immunological assays and other complex medical assays without a laboratory.

Graphical abstract: ELISA-LOC: lab-on-a-chip for enzyme-linked immunodetection

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Publication details

The article was received on 08 Mar 2010, accepted on 18 May 2010 and first published on 11 Jun 2010


Article type: Paper
DOI: 10.1039/C003994B
Lab Chip, 2010,10, 2093-2100

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    ELISA-LOC: lab-on-a-chip for enzyme-linked immunodetection

    S. Sun, M. Yang, Y. Kostov and A. Rasooly, Lab Chip, 2010, 10, 2093
    DOI: 10.1039/C003994B

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