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Issue 20, 2010
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High-speed RNA microextraction technology using magnetic oligo-dT beads and lateral magnetophoresis

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Abstract

This paper presents a high-speed RNA microextractor for the direct isolation of RNA from peripheral blood lysate using magnetic oligo-dT beads. The extraction is achieved through lateral magnetophoresis, generated by a ferromagnetic wire array inlaid on a glass substrate. This RNA microextractor separated more than 80% of magnetic beads with a flow rate up to 20 ml h−1, and the overall extraction procedure was completed within 1 min. The absorbance ratio of RNA to protein (A260/A280) was >1.7, indicating that the extraction technology yielded nearly pure RNA. The feasibility of this technique was evaluated further for its applicability to reverse transcription polymerase chain reaction (RT-PCR) procedures by performing cDNA synthesis and PCR. The analysis verified that the RNA microextractor is a practical method for easy, rapid, and high-precision RT-PCR using minimal reagent volumes without requiring highly trained personnel. In addition, it can be readily incorporated into genetic analysis procedures for realizing automated on-chip genetic platforms in a micro format.

Graphical abstract: High-speed RNA microextraction technology using magnetic oligo-dT beads and lateral magnetophoresis

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Article information


Submitted
19 Apr 2010
Accepted
19 Jul 2010
First published
02 Sep 2010

Lab Chip, 2010,10, 2764-2770
Article type
Paper

High-speed RNA microextraction technology using magnetic oligo-dT beads and lateral magnetophoresis

H. Lee, J. Jung, S. Han and K. Han, Lab Chip, 2010, 10, 2764
DOI: 10.1039/C005145D

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