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Issue 8, 2010
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The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform

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Abstract

We introduce the integration of a novel dielectrophoresis (DEP)-assisted filter with a compact disk (CD)-based centrifugal platform. Carbon-electrode dielectrophoresis (carbon-DEP) refers to the use of carbon electrodes to induce DEP. In this work, 3D carbon electrodes are fabricated using the C-MEMS technique and are used to implement a DEP-enabled active filter to trap particles of interest. Compared to traditional planar metal electrodes, 3D carbon electrodes allow for superior filtering efficiency. The system includes mounting modular 3D carbon-DEP chips on an electrically interfaced rotating disk. This allows simple centrifugal pumping to replace the large footprint syringe pump approaches commonly used in DEP systems. The advantages of the CD setup include not only a reduced footprint, but also complexity and cost reduction by eliminating expensive precision pumps and fluidic interconnects. To demonstrate the viability of this system we quantified the filter efficiency in the DEP trapping of yeast cells from a mix of latex and yeast cells. Results demonstrate selective filtering at flow rates up to 35 µl min−1. The impact of electrode height, DEP chip misalignment and particle sedimentation on filter efficiency and the advantages this system represents are analyzed. The ultimate goal is to obtain an automated platform for bioparticle sorting with application in different fields such as point-of-care diagnostics and cell-based therapies.

Graphical abstract: The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform

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Publication details

The article was received on 02 Dec 2009, accepted on 13 Jan 2010 and first published on 04 Feb 2010


Article type: Paper
DOI: 10.1039/B925456K
Lab Chip, 2010,10, 1030-1043

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    The integration of 3D carbon-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform

    R. Martinez-Duarte, R. A. Gorkin III, K. Abi-Samra and M. J. Madou, Lab Chip, 2010, 10, 1030
    DOI: 10.1039/B925456K

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