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Issue 5, 2010
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Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans

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Abstract

This article describes the fabrication of a microfluidic device for the liquid culture of many individual nematode worms (Caenorhabditis elegans) in separate chambers. Each chamber houses a single worm from the fourth larval stage until death, and enables examination of a population of individual worms for their entire adult lifespans. Adjacent to the chambers, the device includes microfluidic worm clamps, which enable periodic, temporary immobilization of each worm. The device made it possible to track changes in body size and locomotion in individual worms throughout their lifespans. This ability to perform longitudinal measurements within the device enabled the identification of age-related phenotypic changes that correlate with lifespan in C. elegans.

Graphical abstract: Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans

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Publication details

The article was received on 16 Sep 2009, accepted on 24 Nov 2009 and first published on 18 Dec 2009


Article type: Paper
DOI: 10.1039/B919265D
Lab Chip, 2010,10, 589-597

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    Lifespan-on-a-chip: microfluidic chambers for performing lifelong observation of C. elegans

    S. E. Hulme, S. S. Shevkoplyas, A. P. McGuigan, J. Apfeld, W. Fontana and G. M. Whitesides, Lab Chip, 2010, 10, 589
    DOI: 10.1039/B919265D

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