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Issue 35, 2010
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Carbohydrate-coated lanthanide-doped upconverting nanoparticles for lectin recognition

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Abstract

In this study we have developed upconverting lanthanide (Ln3+)-doped nanoparticles conjugated with glycodendrimers capable of recognizing lectins based on luminescence resonance energy transfer (LRET). Poly(amidoamine) (PAMAM) dendrimers were adsorbed on the surface of NaGdF4:Er3+, Yb3+ upconverting nanoparticles (LnNPs) via direct ligand-exchange followed by efficient thiourea linkage formation between the amine surface and p-isothiocyanatophenyl α-D-mannopyranoside for covalent carbohydrate derivatization. The resulting water dispersible and biocompatible mannose-coated PAMAM-LnNPs were used to recognize a lectin, Concanavalin A (a mannose binding protein) conjugated with tetramethylrhodamine (RITC-Con A) via LRET from the upconverting mannose-coated PAMAM-LnNPs, which act as energy donors to the RITC-labeled lectin molecules serving as energy acceptors. The energy transfer phenomenon from the mannose-coated PAMAM-LnNPs, following 980 nm excitation, to the RITC-Con A was observed with increasing emissions at 585 nm, as the concentration of lectin increases. These novel carbohydrate-coated LnNPs prove to be a novel tool to monitor the binding interaction with lectins in aqueous samples.

Graphical abstract: Carbohydrate-coated lanthanide-doped upconverting nanoparticles for lectin recognition

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Publication details

The article was received on 26 May 2010, accepted on 14 Jun 2010 and first published on 02 Aug 2010


Article type: Paper
DOI: 10.1039/C0JM01617A
J. Mater. Chem., 2010,20, 7543-7550

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    Carbohydrate-coated lanthanide-doped upconverting nanoparticles for lectin recognition

    N. Bogdan, F. Vetrone, R. Roy and J. A. Capobianco, J. Mater. Chem., 2010, 20, 7543
    DOI: 10.1039/C0JM01617A

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