Issue 3, 2010
From the journal:

Chemical Society Reviews

DNA in nanochannels—directly visualizing genomic information†

Fredrik Perssona  and  Jonas O. Tegenfeldt*ab  

* Corresponding authors

a University of Gothenburg, Gothenburg, Sweden
E-mail: jonas.tegenfeldt@physics.gu.se

b Lund University, Lund, Sweden

Abstract

The power of nanofluidic channels to analyze DNA is described along with practical experimental hints. As an introduction, a general overview is given on conventional DNA analysis tools, as well as tools under development towards the $1000 genome. The focus of this tutorial review is the stretching of DNA in nanoscale channels for coarse-grained mapping of DNA. To understand the behavior of the DNA, basic theory is discussed. Experimental details are revealed so that the reader, with the proper equipment, should be able to perform experiments. Basic approaches to the analysis of the data are discussed. Finally, potential future directions are discussed including the application of melting mapping as a simple barcode for the DNA.

Graphical abstract: DNA in nanochannels—directly visualizing genomic information

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Article information

DOI
https://doi.org/10.1039/B912918A
Article type
Tutorial Review
Submitted
13 Aug 2009
First published
25 Jan 2010

Chem. Soc. Rev., 2010,39, 985-999

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DNA in nanochannels—directly visualizing genomic information

F. Persson and J. O. Tegenfeldt, Chem. Soc. Rev., 2010, 39, 985 DOI: 10.1039/B912918A

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