Issue 9, 2010
From the journal:

Analytical Methods

Towards an improved qualitative and quantitative determination of glutathione peroxidase, selenoproteinP and selenoalbumin in human serum by HPLC coupled to ICP-MS

Petru Jitaru,*ab   Giulio Cozzi,a   Roberta Seraglia,c   Pietro Traldi,c   Paolo Cesconad  and  Carlo Barbantead  

* Corresponding authors

a Institute for the Dynamics of Environmental Processes (IDPA-CNR), Dorsoduro 2137, Venice, Italy

b Institut Polytechnique LaSalle Beauvais, Département des Sciences et Techniques Agro-Industrielles, 19 rue Pierre Waguet BP 30313, 60026 Beauvais Cedex, France
E-mail: Petru.Jitaru@lasalle-beauvais.fr
Fax: + 33 3 44 06 25 26
Tel: + 33 3 44 06 89 72

c Institute of Scientific and Molecular Technologies (ISTM-CNR), Corso Stati Uniti 4, Padova, Italy

d University of Venice Ca' Foscari, Department of Environmental Sciences, Dorsoduro 2137, Venice, Italy

Abstract

This paper deals primarily with the validation of a clean-up procedure based on anion exchange solid-phase extraction for the accurate determination of glutathione peroxidase (GPx), selenoprotein P (SelP) and selenoalbumin (SeAlb) in human serum by affinity HPLC (AF-HPLC) coupled to inductively coupled plasma-mass spectrometry (ICP-MS). In addition, the identification and the purity assessment of the GPx, SelP and SeAlb peak fractions separated by AF-HPLC is addressed by their analysis using matrix assisted laser desorption ionization-time of flight mass spectrometry.

Graphical abstract: Towards an improved qualitative and quantitative determination of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by HPLC coupled to ICP-MS

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Article information

DOI
https://doi.org/10.1039/C0AY00173B
Article type
Technical Note
Submitted
15 Mar 2010
Accepted
27 Jun 2010
First published
12 Jul 2010

Anal. Methods, 2010,2, 1382-1387

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Towards an improved qualitative and quantitative determination of glutathione peroxidase, selenoprotein P and selenoalbumin in human serum by HPLC coupled to ICP-MS

P. Jitaru, G. Cozzi, R. Seraglia, P. Traldi, P. Cescon and C. Barbante, Anal. Methods, 2010, 2, 1382 DOI: 10.1039/C0AY00173B

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