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Issue 12, 2010
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Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy

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Abstract

FTIR and Raman spectromicroscopy were used to characterize the composition of Curvularia protuberata hyphae, and to compare a strain isolated from plants inhabiting geothermal soils with a non-geothermal isolate. Thermal IR source images of hyphae have been acquired with a 64 × 64 element focal plane array detector; single point IR spectra have been obtained with synchrotron source light. In some C. protuberata hyphae, we have discovered the spectral signature of crystalline mannitol, a fungal polyol with complex protective roles. With FTIR-FPA imaging, we have determined that the protein content in cells remains fairly constant throughout the length of a hypha, whereas the mannitol is found at discrete, irregular locations. This is the first direct observation of mannitol in intact fungal hyphae. Since the concentration of mannitol in cells varies with respect to position and is not present in all hyphae, this discovery may be related to habitat adaptation, fungal structure and growth stages.

Graphical abstract: Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy

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Article information


Submitted
16 Jul 2010
Accepted
21 Sep 2010
First published
21 Oct 2010

Analyst, 2010,135, 3249-3254
Article type
Paper

Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy

M. Isenor, S. G. W. Kaminskyj, R. J. Rodriguez, R. S. Redman and K. M. Gough, Analyst, 2010, 135, 3249
DOI: 10.1039/C0AN00534G

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