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Issue 11, 2010
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Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3

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Abstract

This paper describes the development and preliminary testing of a competitive surface-enhanced Raman scattering (SERS) immunoassay for calcitriol, the 1,25-dihydroxy metabolite (1,25-(OH)2-D3) of vitamin D3. Deficiencies in 1,25-(OH)2-D have been linked to renal disease, while elevations are linked to hypercalcemia. Thus, there has been a sharp increase in the clinical demand for measurements of this metabolite. The work herein extends the many attributes of SERS-based sandwich immunoassays that have been exploited extensively in the detection of large biolytes (e.g., DNA, proteins, viruses, and microorganisms) into a competitive immunoassay for the low level determination of a small biolyte, 1,25-(OH)2-D3 (Mw = 416 g mol−1). The assay uses surface modified gold nanoparticles as SERS labels, and has a dynamic range of 10–200 pg mL−1 and a limit of detection of 8.4 ± 1.8 pg mL−1. These analytical performance metrics match those of tests for 1,25-(OH)2-D3 that rely on radio- or enzyme-labels, while using a much smaller sample volume and eliminating the disposal of radioactive wastes. Moreover, the SERS-based data from pooled-patient sera show strong agreement with that from radioimmunoassays. The merits and potential utility of this new assay are briefly discussed.

Graphical abstract: Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3

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Article information


Submitted
26 May 2010
Accepted
25 Jul 2010
First published
07 Sep 2010

Analyst, 2010,135, 2811-2817
Article type
Paper

Competitive surface-enhanced Raman scattering assay for the 1,25-dihydroxy metabolite of vitamin D3

E. J. Dufek, B. Ehlert, M. C. Granger, T. M. Sandrock, S. L. Legge, M. G. Herrmann, A. W. Meikle and M. D. Porter, Analyst, 2010, 135, 2811
DOI: 10.1039/C0AN00354A

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