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Issue 8, 2010
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A cyclic enzymatic amplification method for sensitive and selective detection ofnucleic acids

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Abstract

Based on Exonuclease III (Exo III) and displacing probes, we have developed a Cyclic Enzymatic Amplification Method (CEAM) for sensitive and selective detection of nucleic acids. In this design, the displacing probe is non-fluorescent on its own and cannot be digested by Exo III until displacement hybridization by a target sequence, leading to release of free non-quenched fluorophore. Because a single target sequence can lead to the release and digestion of numerous fluorophore strands from the displacing probe, a remarkable signal amplification is achieved. With this method, DNA can be detected in the picomolar range with a high selectivity and within less than 20 min.

Graphical abstract: A cyclic enzymatic amplification method for sensitive and selective detection of nucleic acids

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Article information


Submitted
07 Apr 2010
Accepted
07 May 2010
First published
18 Jun 2010

Analyst, 2010,135, 2069-2073
Article type
Paper

A cyclic enzymatic amplification method for sensitive and selective detection of nucleic acids

L. Cui, G. Ke, C. Wang and C. J. Yang, Analyst, 2010, 135, 2069 DOI: 10.1039/C0AN00215A

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