Issue 11, 2024

Protective effects of EGCG on acrolein-induced Caenorhabditis elegans and its mechanism of life extension

Abstract

In this study, it was found that epigallocatechin-3-gallate (EGCG) could extend the lifespan of Caenorhabditis elegans (C. elegans) induced by 100 μM acrolein (ACR) at all test concentrations (300, 400, 500, 600, and 700 μM). Notably, 500 μM EGCG exhibited the most significant mean lifespan extension, increasing it by approximately 32.5%. Furthermore, 500 μM EGCG effectively reduced elevated levels of reactive oxygen species (ROS) and lipofuscin production caused by acrolein. It also bolstered the activity of antioxidant enzymes and mitigated malondialdehyde (MDA) levels compared to the ACR-only group. These effects appeared independent of dietary restrictions. Additionally, qPCR results revealed different changes in the transcription levels of 11 genes associated with antioxidative and anti-aging functions following EGCG treatment. At the expression level, GST-4::GFP, SOD-3::GFP and HSP-16.2::GFP exhibited an initial increase with ACR treatment followed by a decrease with EGCG treatment, while the expression pattern of these three GFPs remained consistent with the enzyme activity and transcription regulation level. EGCG treatment also reduced the nuclear localization of SKN-1 and DAF-16 in the MAPK and IIS pathways that were enhanced by ACR. Moreover, the longevity-promoting effects of EGCG were diminished or absent in 13 longevity gene-deletion mutants. In conclusion, EGCG demonstrates protective effects on ACR-induced C. elegans, with the IIS and MAPK pathways playing a critical role in enhancing resilience to ACR.

Graphical abstract: Protective effects of EGCG on acrolein-induced Caenorhabditis elegans and its mechanism of life extension

Supplementary files

Article information

Article type
Paper
Submitted
06 Dec 2023
Accepted
17 Apr 2024
First published
17 Apr 2024

Food Funct., 2024,15, 5855-5867

Protective effects of EGCG on acrolein-induced Caenorhabditis elegans and its mechanism of life extension

Y. Song, D. Liu, J. Xie, J. Xie, Y. Chen, X. Chen, X. Hu and Q. Yu, Food Funct., 2024, 15, 5855 DOI: 10.1039/D3FO05394F

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