Issue 7, 2009
From the journal:

Organic & Biomolecular Chemistry

Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase†

Rakesh N. Veedu,a   Birte Vestera  and  Jesper Wengel*a  

* Corresponding authors

a Nucleic Acid Center, Department of Physics and Chemistry and Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, Denmark
E-mail: jwe@ifk.sdu.dk
Fax: +45 6550 2467
Tel: +45 6550 2377

Abstract

Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.

Graphical abstract: Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

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Article information

DOI
https://doi.org/10.1039/B819946A
Article type
Paper
Submitted
07 Nov 2008
Accepted
21 Jan 2009
First published
24 Feb 2009

Org. Biomol. Chem., 2009,7, 1404-1409

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Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase

R. N. Veedu, B. Vester and J. Wengel, Org. Biomol. Chem., 2009, 7, 1404 DOI: 10.1039/B819946A

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