A microfabricated device has been developed for the selective detection of lead in water. It is based on the use of a selective and sensitive fluorescent molecular sensor for lead (Calix-DANS4) which contains a calix[4]arene bearing four dansyl groups. The microchip-based lead sensor contains a Y-shape microchannel equipped with a passive mixer and moulded on a glass substrate. An optimization of the microcircuit length has been performed in order to have a full complexation of the Calix-DANS4. The detection is performed by using a configuration in which the sensing molecules are excited by two optical fibres, each one connected to a 365 nm UV LED, and the light collection is made by another optical fibre with a photomultiplier. By using this configuration we have shown the possibility to detect lead with a detection limit of 5 ppb. The effect of interfering cations such as calcium has been evaluated. The obtained measurements have been validated by an alternative method (ASV).
