Issue 8, 2009
From the journal:

Lab on a Chip

Controlling the selection stringency of phage display using a microfluidic device

Yanli Liu,ab   Jonathan D. Adams,c   Kelisha Turner,e   Frank V. Cochran,f   Sanjiv Sam Gambhirf  and  H. Tom Soh*bd  

* Corresponding authors

a Neuroscience Research Institute, University of California, Santa Barbara, CA, USA

b Department of Materials, University of California, Santa Barbara, CA, USA
E-mail: tsoh@engineering.ucsb.edu
Fax: +1-805-893-8651
Tel: +1-805-893-8737

c Department of Physics, University of California, Santa Barbara, CA, USA

d Department of Mechanical Engineering, University of California, Santa Barbara, CA, USA

e School of Science and Technology, Jackson State University, Jackson, MS, USA

f Molecular Imaging Program at Stanford, Department of Radiology & Bio-X Program, Stanford University, Stanford, CA, USA

Abstract

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

Graphical abstract: Controlling the selection stringency of phage display using a microfluidic device

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Article information

DOI
https://doi.org/10.1039/B820985E
Article type
Communication
Submitted
24 Nov 2008
Accepted
18 Feb 2009
First published
03 Mar 2009

Lab Chip, 2009,9, 1033-1036

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Controlling the selection stringency of phage display using a microfluidic device

Y. Liu, J. D. Adams, K. Turner, F. V. Cochran, S. S. Gambhir and H. T. Soh, Lab Chip, 2009, 9, 1033 DOI: 10.1039/B820985E

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