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Issue 5, 2009
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Antigen detection using polymerization-based amplification

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Abstract

The influenza virus has been subtyped from crude lysates using polymerization-based amplification. In this novel chemical approach to detection, signal amplification was achieved by coupling a polymerization reaction to a proteinprotein recognition event. This particular method shows promise due to its advantages over the techniques currently employed in commercial assays in terms of cost, robustness, and unambiguity of test results. Instrumentation is not required to see the crosslinked hydrogel “readout”, and no false positives or false negatives were observed above the limit of detection.

Graphical abstract: Antigen detection using polymerization-based amplification

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Supplementary files

Article information


Submitted
16 Sep 2008
Accepted
20 Nov 2008
First published
08 Dec 2008

Lab Chip, 2009,9, 653-656
Article type
Communication

Antigen detection using polymerization-based amplification

H. D. Sikes, R. Jenison and C. N. Bowman, Lab Chip, 2009, 9, 653 DOI: 10.1039/B816198D

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