Owing to the color (blue-to-red) and fluorescence (non-to-fluorescent) changes that take place in response to environmental perturbations, conjugated polydiacetylenes (PDAs) have been actively employed as sensory materials for the detection of biologically-, environmentally- and chemically-important target molecules. Until recently, the majority of PDA sensors have been prepared in the form of aqueous suspensions or Langmuir-type thin films on solid substrates. In order to overcome the limitations associated with conventional solution/film sensors, conceptually new formats, such as immobilized PDAs in and on solid substrates, microarrayed PDA sensors, microfluidic PDA sensors, as well as PDA-embedded electrospun fiber sensors and resonance energy transfer (RET)-based PDA sensors, have been developed recently. In this tutorial review, the recent conceptual and technological achievements made in the area of conjugated PDA chemosensors are described.
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