Issue 7, 2009
From the journal:

Analyst

An operationally simple colorimetric assay of hyaluronidase activity using cationic gold nanoparticles†‡

Jung-Won Kim ,§a   Joong Hyun Kim,a   Sang Jeon Chungab  and  Bong Hyun Chung*ab  

* Corresponding authors

a BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong-gu, Daejeon, South Korea
E-mail: chungbh@kribb.re.kr
Fax: + 82-42-879-8594
Tel: + 82-42-860-4442

b School of Engineering, University of Science and Technology, P.O. Box 115, Yuseong-gu, Daejeon, South Korea

Abstract

An operationally simple colorimetric way for measuring hyaluronidase activity was developed using cysteamine-bound gold nanoparticles. The addition of gold nanoparticles into hyaluronidase-containing solutions resulted in color changes, which could easily be observed with the naked eye or a UV/Vis spectrophotometer.

Graphical abstract: An operationally simple colorimetric assay of hyaluronidase activity using cationic gold nanoparticles

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Article information

DOI
https://doi.org/10.1039/B822917A
Article type
Communication
Submitted
19 Dec 2008
Accepted
23 Mar 2009
First published
27 Mar 2009

Analyst, 2009,134, 1291-1293

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An operationally simple colorimetric assay of hyaluronidase activity using cationic gold nanoparticles

J. Kim , J. H. Kim, S. J. Chung and B. H. Chung, Analyst, 2009, 134, 1291 DOI: 10.1039/B822917A

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