Jump to main content
Jump to site search

Issue 10, 2009
Previous Article Next Article

Bursting of charged multicomponent vesicles subjected to electric pulses

Author affiliations

Abstract

Strong electric pulses applied to phosphatidylcholine (PC) giant vesicles induce the formation of pores, which reseal within milliseconds. Here, we study the response of vesicles containing PC and negatively charged lipids like phospatidylglycerol (PG) to such pulses. Vesicles composed of 1:1 PG:PC in a buffered solution of Hepes and EDTA exhibit the same behaviour as observed with PC membranes. Surprisingly, when the medium is changed to a non-buffered solution with or without salt, the vesicles burst and disintegrate to tubular structures after the pulse is applied. A fast digital camera and confocal microscopy were used to observe the dynamics of vesicle rupture and the membrane reorganization after the applied pulse. The nature of this instability and structural rearrangement is poorly understood. The experiments reported here suggest that the membrane charge plays a significant role. Vesicles made of lipid extract from human plasma membranes behave in the same fashion, implying that the reported bilayer reorganization may also occur to a certain degree in the membrane of electroporated cells.

Graphical abstract: Bursting of charged multicomponent vesicles subjected to electric pulses

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Jan 2009, accepted on 25 Mar 2009 and first published on 02 Apr 2009


Article type: Communication
DOI: 10.1039/B900548J
Citation: Soft Matter, 2009,5, 1983-1986
  •   Request permissions

    Bursting of charged multicomponent vesicles subjected to electric pulses

    K. A. Riske, R. L. Knorr and R. Dimova, Soft Matter, 2009, 5, 1983
    DOI: 10.1039/B900548J

Search articles by author

Spotlight

Advertisements