Jump to main content
Jump to site search

Issue 2, 2009
Previous Article Next Article

Development and application of a novel acridinium ester for use as a chemiluminescent emitter in nucleic acid hybridisation assays using chemiluminescence quenching

Author affiliations

Abstract

Chemiluminescent acridinium esters (AEs) permit the development of high sensitivity ligand binding assays due to a combination of high intensity light emission and very low backgrounds. Here these advantages are exploited for use in homogeneous nucleic acid hybridisation assays using quenched chemiluminescence. AE chemiluminescence is conventionally initiated at highly alkaline pH. Novel “active” AEs were designed that permit initiation under conditions compatible with maintenance of nucleic acid hybrids (i.e. pH less than 9). Methyl red was found to be a dark quencher species capable of functioning at this pH. Practical application of the chemiluminescence quenching assay system has been demonstrated using two model nucleic acid hybridisation assays based on intra- and intermolecular emitter/quencher pairs.

Graphical abstract: Development and application of a novel acridinium ester for use as a chemiluminescent emitter in nucleic acid hybridisation assays using chemiluminescence quenching

Back to tab navigation

Article information


Submitted
14 Jul 2008
Accepted
21 Oct 2008
First published
24 Nov 2008

Org. Biomol. Chem., 2009,7, 386-394
Article type
Paper

Development and application of a novel acridinium ester for use as a chemiluminescent emitter in nucleic acid hybridisation assays using chemiluminescence quenching

R. C. Brown, Z. Li, A. J. Rutter, X. Mu, O. H. Weeks, K. Smith and I. Weeks, Org. Biomol. Chem., 2009, 7, 386
DOI: 10.1039/B811947C

Social activity

Search articles by author

Spotlight

Advertisements