Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

There will be scheduled maintenance work beginning on Saturday 15th June 2019 at 8:30 am through to Sunday 16th June 2019 at 11:30 pm (BST).

During this time our website may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 13, 2009
Previous Article Next Article

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

Author affiliations

Abstract

We describe a highly efficient microfluidic fluorescence-activated droplet sorter (FADS) combining many of the advantages of microtitre-plate screening and traditional fluorescence-activated cell sorting (FACS). Single cells are compartmentalized in emulsion droplets, which can be sorted using dielectrophoresis in a fluorescence-activated manner (as in FACS) at rates up to 2000 droplets s−1. To validate the system, mixtures of E. colicells, expressing either the reporter enzyme β-galactosidase or an inactive variant, were compartmentalized with a fluorogenic substrate and sorted at rates of ∼300 droplets s−1. The false positive error rate of the sorter at this throughput was <1 in 104 droplets. Analysis of the sorted cells revealed that the primary limit to enrichment was the co-encapsulation of E. colicells, not sorting errors: a theoretical model based on the Poisson distribution accurately predicted the observed enrichment values using the starting cell density (cells per droplet) and the ratio of active to inactive cells. When the cells were encapsulated at low density (∼1 cell for every 50 droplets), sorting was very efficient and all of the recovered cells were the active strain. In addition, single active droplets were sorted and cells were successfully recovered.

Graphical abstract: Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Feb 2009, accepted on 14 Apr 2009 and first published on 23 Apr 2009


Article type: Paper
DOI: 10.1039/B902504A
Lab Chip, 2009,9, 1850-1858

  •   Request permissions

    Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

    J. Baret, O. J. Miller, V. Taly, M. Ryckelynck, A. El-Harrak, L. Frenz, C. Rick, M. L. Samuels, J. B. Hutchison, J. J. Agresti, D. R. Link, D. A. Weitz and A. D. Griffiths, Lab Chip, 2009, 9, 1850
    DOI: 10.1039/B902504A

Search articles by author

Spotlight

Advertisements