Jump to main content
Jump to site search

Issue 8, 2009
Previous Article Next Article

Controlling the selection stringency of phage display using a microfluidic device

Author affiliations

Abstract

We report the utilization of microfluidic technology to phage selection and demonstrate that accurate control of washing stringency in our microfluidic magnetic separator (MMS) directly impacts the diversity of isolated peptide sequences. Reproducible generation of magnetic and fluidic forces allows controlled washing conditions that enable rapid convergence of selected peptide sequences. These findings may provide a foundation for the development of automated microsystems for rapid in vitro directed evolution of affinity reagents.

Graphical abstract: Controlling the selection stringency of phage display using a microfluidic device

Back to tab navigation

Additions and corrections

Article information


Submitted
24 Nov 2008
Accepted
18 Feb 2009
First published
03 Mar 2009

Lab Chip, 2009,9, 1033-1036
Article type
Communication

Controlling the selection stringency of phage display using a microfluidic device

Y. Liu, J. D. Adams, K. Turner, F. V. Cochran, S. S. Gambhir and H. T. Soh, Lab Chip, 2009, 9, 1033 DOI: 10.1039/B820985E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements