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Issue 11, 2009
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Integrated microfluidic immunoassay for the rapid determination of clenbuterol

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Abstract

An integrated microfluidic immunoassay system was established for high throughput analysis of clenbuterol. This system consisted of an integrated microchip and a linear confocal laser induced fluorescence (LIF) scanner. The microchip was composed of three layers: a fluidic channel layer, a PDMS membrane layer and a pneumatic control layer. The multi-layer chip was integrated with 36 pneumatic micro-valves and multiple micro-pumps to realize the flexible reagent delivery, facilitating the automatic assays with less consumption of samples and reduced analysis time. The homemade LIF scanner was able to simultaneously detect multi-channels and provide the potential capability of high throughput assays. The performance of the system was demonstrated by the determination of clenbuterol, one of the most widely used β-agonists. Under the optimal conditions, the linear range and the limit of detection of clenbuterol were 0∼5.0 ng mL−1 and 0.088 ng mL−1, respectively. The recovery rates determined with pig urine samples of 1.0 ng mL−1 and 2.0 ng mL−1 were 98.74% and 102.51% (n = 3), respectively. The total detection time was less than 30 min. The system had the potential application for rapid detection of multiple β-agonists in clinical, pharmaceutical and chemical analyses.

Graphical abstract: Integrated microfluidic immunoassay for the rapid determination of clenbuterol

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Publication details

The article was received on 17 Oct 2008, accepted on 10 Feb 2009 and first published on 03 Mar 2009


Article type: Paper
DOI: 10.1039/B818430E
Lab Chip, 2009,9, 1541-1547

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    Integrated microfluidic immunoassay for the rapid determination of clenbuterol

    J. Kong, L. Jiang, X. Su, J. Qin, Y. Du and B. Lin, Lab Chip, 2009, 9, 1541
    DOI: 10.1039/B818430E

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