Jump to main content
Jump to site search

Issue 7, 2009
Previous Article Next Article

A fritless, EOF microchip pump for high pressure pumping of aqueous and organic solvents

Author affiliations

Abstract

A fritless, microchip electroosmotic flow (EOF) pump is microfabricated and demonstrated on a planar soda lime glass substrate to be capable of supplying reasonable flow rates under high back pressures, such as that required for micro-high pressure liquid chromatography (µ-HPLC). The microchip EOF pump is composed of a densely packed microchannel containing 800 nm silica particles and was capable of generating a maximum pressure > 1000 psi (∼ 7 MPa) and a maximum flow rate of 282 nL/min (aqueous cyclohexylamino alkyl sulfonate (CHES) buffer, 10 mM, pH 9.0, 200 V/cm). Other pumping fluids, such as CHES bufferacetonitrile mixture (50%, v/v), CHES buffermethanol mixture (50%, v/v), and pure acetonitrile were also used in a characterization of pump performance that included determinations of the maximum flow rate, maximum pressure, and resulting flow rate against an applied, downstream back pressure. The flow rate under a 200 psi (∼1.4 MPa) back pressure at an applied electric field strength of 250 V/cm ranged from 285 nL/min for aqueous CHES buffer to 44 nL/min for CHES bufferacetonitrile mixture (50%, v/v), indicating that this EOF pump will meet the future requirements of a µ-HPLC system.

Graphical abstract: A fritless, EOF microchip pump for high pressure pumping of aqueous and organic solvents

Back to tab navigation

Article information


Submitted
18 Sep 2008
Accepted
11 Dec 2008
First published
13 Jan 2009

Lab Chip, 2009,9, 954-960
Article type
Paper

A fritless, EOF microchip pump for high pressure pumping of aqueous and organic solvents

Q. Lu and G. E. Collins, Lab Chip, 2009, 9, 954
DOI: 10.1039/B816291C

Social activity

Search articles by author

Spotlight

Advertisements