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Issue 18, 2009
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The microfluidic palette: A diffusive gradient generator with spatio-temporal control

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Abstract

This paper describes a new microfluidic device called the “microfluidic palette”, capable of generating multiple spatial chemical gradients simultaneously inside a microfluidic chamber. The unique aspect of this work is that chemical gradients are generated by diffusion, without convection, and can either be held constant over long periods, or modified dynamically. We characterized a representative device with a 1.5 mm circular chamber where diffusion takes place, and three access-ports for the delivery and removal of solutes. The gradient stabilizes in ≈ 15 min for small molecules and can be maintained constant indefinitely. We demonstrate overlapping gradients with different spatial location and a controlled rotation of a diffusive gradient around its centre. Finally, we demonstrate the applicability of this tool to study the chemotactic response of the bacteria P. aeruginosa to glucose.

Graphical abstract: The microfluidic palette: A diffusive gradient generator with spatio-temporal control

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Supplementary files

Article information


Submitted
05 Feb 2009
Accepted
03 Jun 2009
First published
22 Jun 2009

Lab Chip, 2009,9, 2707-2714
Article type
Paper

The microfluidic palette: A diffusive gradient generator with spatio-temporal control

J. Atencia, J. Morrow and L. E. Locascio, Lab Chip, 2009, 9, 2707 DOI: 10.1039/B902113B

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