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Issue 4, 2009
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An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells

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Abstract

We have successfully designed and fabricated an integrated microfluidic platform, the hESC-µChip, which is capable of reproducible and quantitative culture and analysis of individual hESC colonies in a semi-automated fashion. In this device, a serpentine microchannel allows pre-screening of dissociated hESC clusters, and six individually addressable cell culture chambers enable parallel hESC culture, as well as multiparameter analyses in sequence. In order to quantitatively monitor hESC proliferation and pluripotency status in real time, knock-in hESC lines with EGFP driven by the endogenous OCT4 promoter were constructed. On-chip immunoassays of several pluripotency markers were carried out to confirm that the hESC colonies maintained their pluripotency. For the first time, our studies demonstrated well characterized hESC culture and analysis in a microfluidic setting, as well as a proof-of-concept demonstration of parallel/multiparameter/real-time/automated examination of self-renewal and differentiation in the same device.

Graphical abstract: An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells

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Supplementary files

Article information


Submitted
29 May 2008
Accepted
14 Oct 2008
First published
20 Nov 2008

Lab Chip, 2009,9, 555-563
Article type
Paper

An integrated microfluidic culture device for quantitative analysis of human embryonic stem cells

K. Kamei, S. Guo, Z. T. F. Yu, H. Takahashi, E. Gschweng, C. Suh, X. Wang, J. Tang, J. McLaughlin, O. N. Witte, K. Lee and H. Tseng, Lab Chip, 2009, 9, 555
DOI: 10.1039/B809105F

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