Jump to main content
Jump to site search

Issue 1, 2009
Previous Article Next Article

CO2 and compressive immobilization of C. elegans on-chip

Author affiliations

Abstract

We present two microfluidic approaches for immobilizing the roundworm C. elegans on-chip. The first approach creates a CO2 micro-environment while the second one utilizes a deformable PDMS membrane to mechanically restrict the worm's movement. An on-chip ‘behavior’ module was used to characterize the effect of these methods on the worm's locomotion pattern. Our results indicate that both methods are appropriate for the short-term (minutes) worm immobilization. The CO2 method offers the additional advantages of long-term immobilization (1–2 hours) and reduced photobleaching, if fluorescent imaging during immobilization is required. We envision the use of these methods in a wide variety of biological studies in C. elegans, including cell developmental and neuronal regeneration studies.

Graphical abstract: CO2 and compressive immobilization of C. elegans on-chip

Back to tab navigation

Supplementary files

Article information


Submitted
30 Apr 2008
Accepted
02 Sep 2008
First published
21 Oct 2008

Lab Chip, 2009,9, 151-157
Article type
Paper

CO2 and compressive immobilization of C. elegans on-chip

T. V. Chokshi, A. Ben-Yakar and N. Chronis, Lab Chip, 2009, 9, 151
DOI: 10.1039/B807345G

Social activity

Search articles by author

Spotlight

Advertisements