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Issue 20, 2009
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Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

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Abstract

A novel method for quantifying biomolecules immobilised onto gold and silver nanoparticles is reported; fluorescent-labelled antibodies and DNA are hydrolysed on the surface of the nanoparticles by the addition of trypsin and DNase I, respectively, resulting in the release of the quantifiable fluorescent label into the bulk solution.

Graphical abstract: Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

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Supplementary files

Article information


Submitted
23 Dec 2008
Accepted
16 Mar 2009
First published
31 Mar 2009

Chem. Commun., 2009, 2872-2874
Article type
Communication

Quantitation of biomolecules conjugated to nanoparticles by enzyme hydrolysis

F. McKenzie, V. Steven, A. Ingram and D. Graham, Chem. Commun., 2009, 2872
DOI: 10.1039/B823057A

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