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Issue 8, 2009
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Labeling strategies in mass spectrometry-based protein quantitation

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Abstract

Mass spectrometry-based techniques for relative and absolute protein quantitation have advanced greatly over the past decade. New measurement strategies and improvements to existing methodologies have expanded the utility of mass spectrometry-based characterization of protein expression profiles, in particular by targeting a diverse array of chemical functionality (e.g. post-translational modifications, specific amino acid residues, etc.) by selective stable isotope incorporation. This article focuses on current trends in stable isotope labeling for protein quantitation using enzymatic, metabolic, and chemical derivatization techniques. Furthermore, new advances in phosphoproteomic labeling and lanthanide-based labeling methods are described.

Graphical abstract: Labeling strategies in mass spectrometry-based protein quantitation

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Article information


First published
09 Jun 2009

Analyst, 2009,134, 1525-1530
Article type
Minireview

Labeling strategies in mass spectrometry-based protein quantitation

R. L. Gant-Branum, T. J. Kerr and J. A. McLean, Analyst, 2009, 134, 1525
DOI: 10.1039/B904643G

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