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Issue 1, 2009
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Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors

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Abstract

An extremely sensitive procedure for the isolation and detection of DNA from bacterial cell cultures is described. Direct asymmetric PCR amplified products from E. coli cultures are specifically detected, at a concentration level as low as 0.01 cfu mL−1 (cfu, colony forming unit), using disposable magnetic DNA hybridization amperometric sensors with no need for culture preconcentration steps.

Graphical abstract: Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors

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Article information


Submitted
02 Sep 2008
Accepted
23 Oct 2008
First published
04 Nov 2008

Analyst, 2009,134, 34-37
Article type
Communication

Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors

Ó. A. Loaiza, S. Campuzano, M. Pedrero, P. García and J. M. Pingarrón, Analyst, 2009, 134, 34
DOI: 10.1039/B815307H

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