Issue 3, 2009
From the journal:

Analyst

Elemental bio-imaging of melanoma in lymph node biopsies

Dominic Hare,a   Fiona Burger,a   Christine Austin,a   Fred Fryer,b   Rudolf Grimm,c   Brian Reedy,a   Richard A Scolyer,d   John F. Thompsond  and  Philip Doble*a  

* Corresponding authors

a Elemental Bio-imaging Facility, University of Technology, Department of Chemistry and Forensic Science, Sydney, New South Wales, Australia
E-mail: philip.doble@uts.edu.au
Fax: +61 2 9514 1460
Tel: +61 2 9514 1792

b Agilent Technologies Australia, North Ryde, New South Wales, Australia

c Agilent Technologies Inc., Santa Clara, California, USA

d Sydney Melanoma Unit and Melanoma Institute Australia, Royal Prince Alfred and Mater Hospitals, Sydney, New South Wales, Australia

Abstract

The spatial distribution of trace elements in human lymph nodes partially infiltrated by melanoma cells was determined by elemental bio-imaging. Imaging of 31P within the nodal capsule and normal lymph node tissue showed a clear demarcation of the tumour boundary, with a significant decrease in relative 31P concentration within the tumour. The location of the tumour boundary was confirmed by haematoxylin and eosin staining of serial sections and observation by light microscopy. Further enhancement of the tumour boundary was achieved by imaging the 31P/34S ratio. 31P/66Zn ratio images showed a decreasing ratio beyond the tumour boundary that extended into peritumour normal lymph node tissue.

Graphical abstract: Elemental bio-imaging of melanoma in lymph node biopsies

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Article information

DOI
https://doi.org/10.1039/B812745J
Article type
Paper
Submitted
24 Jul 2008
Accepted
07 Nov 2008
First published
27 Nov 2008

Analyst, 2009,134, 450-453

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Elemental bio-imaging of melanoma in lymph node biopsies

D. Hare, F. Burger, C. Austin, F. Fryer, R. Grimm, B. Reedy, R. A. Scolyer, J. F. Thompson and P. Doble, Analyst, 2009, 134, 450 DOI: 10.1039/B812745J

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