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Issue 6, 2008
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Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellularpH measurements

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Abstract

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.

Graphical abstract: Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

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Article information


Submitted
09 Jan 2008
Accepted
19 Mar 2008
First published
01 Apr 2008

Photochem. Photobiol. Sci., 2008,7, 668-670
Article type
Communication

Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements

T. Nakabayashi, H. Wang, M. Kinjo and N. Ohta, Photochem. Photobiol. Sci., 2008, 7, 668
DOI: 10.1039/B800391B

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