Jump to main content
Jump to site search

Issue 20, 2008
Previous Article Next Article

Oligonucleotide conjugation to a cell-penetrating (TAT) peptide by Diels–Alder cycloaddition

Author affiliations

Abstract

Modifed oligonucleotides are routinely employed as analytical probes for use in diagnostics, e.g. in the examination of specific RNA sequences for infectious diseases, however, a major limiting factor in oligonucleotide-based diagnostics is poor cellular uptake of naked oligonucleotides. This problem can be overcome by covalent attachment of a so-called ‘cell-penetrating peptide’ to form an oligonucleotide peptide conjugate. Stepwise solid phase synthesis of such a conjugate is difficult and expensive due to the conflicting chemistries of oligonucleotides and peptides. A simple approach to overcome this is post-synthetic conjugation. Diels–Alder cycloaddition is an attractive methodology for oligonucleotide peptide conjugation; the reaction is fast, chemoselective and the reaction rate is greatly enhanced in aqueous media – ideal conditions for biological moieties. An oligodeoxyribonucleotide sequence has been derivatised with a series of dienes at the 5′-terminus, using a series of unique dienyl-modified phosphoramidites, and investigation into the effect of diene type on the efficiency of conjugation, using Diels–Alder cycloaddition with a maleimido-derivatised cell-penetrating (TAT) peptide, has been performed. This led to the observation that the optimal diene for conjugation was cyclohexadiene, allowing conjugation of oligodeoxyribonucleotides to a cell-penetrating peptide by Diels–Alder cycloaddition for the first time.

Graphical abstract: Oligonucleotide conjugation to a cell-penetrating (TAT) peptide by Diels–Alder cycloaddition

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 May 2008, accepted on 17 Jul 2008 and first published on 26 Aug 2008


Article type: Paper
DOI: 10.1039/B807843B
Org. Biomol. Chem., 2008,6, 3781-3787

  •   Request permissions

    Oligonucleotide conjugation to a cell-penetrating (TAT) peptide by Diels–Alder cycloaddition

    V. Steven and D. Graham, Org. Biomol. Chem., 2008, 6, 3781
    DOI: 10.1039/B807843B

Search articles by author

Spotlight

Advertisements