Issue 6, 2008

Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues

Abstract

Feeding 5-hydroxy and 5-fluorotryptophan to a Streptomyces coelicolorTrp-auxotrophic strain WH101 results in the production of a number of new calcium-dependent antibiotics (CDAs) possessing modified Trp residues. It is anticipated that this method could be used to modulate the biological properties of Trp-containing nonribosomal peptide natural products, or to generate analogues with useful fluorescent properties for studying biological mechanisms of action.

Graphical abstract: Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues

Supplementary files

Article information

Article type
Communication
Submitted
04 Dec 2007
Accepted
18 Jan 2008
First published
11 Feb 2008

Org. Biomol. Chem., 2008,6, 975-978

Auxotrophic-precursor directed biosynthesis of nonribosomal lipopeptides with modified tryptophan residues

B. Amir-Heidari, J. Thirlway and J. Micklefield, Org. Biomol. Chem., 2008, 6, 975 DOI: 10.1039/B718766C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements