Jump to main content
Jump to site search

Issue 12, 2008
Previous Article Next Article

Patterningprotein concentration using laser-assisted adsorption by photobleaching, LAPAP

Author affiliations

Abstract

The study of cellular responses to changes in the spatial distribution of molecules in development, immunology and cancer, requires reliable methods to reproduce in vitro the precise distributions of proteins found in vivo. Here we present a straightforward method for generating substrate-bound protein patterns which has the simplicity required to be implemented in typical life science laboratories. The method exploits photobleaching of fluorescently tagged molecules to generate patterns and concentration gradients of protein with sub-micron spatial resolution. We provide an extensive characterization of the technique and demonstrate, as proof of principle, axon guidance by gradients of substrate-bound laminin peptide generated in vitro using LAPAP.

Graphical abstract: Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP

Back to tab navigation

Supplementary files

Article information


Submitted
11 Aug 2008
Accepted
03 Oct 2008
First published
17 Oct 2008

Lab Chip, 2008,8, 2164-2167
Article type
Communication

Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP

J. M. Bélisle, J. P. Correia, P. W. Wiseman, T. E. Kennedy and S. Costantino, Lab Chip, 2008, 8, 2164
DOI: 10.1039/B813897D

Social activity

Search articles by author

Spotlight

Advertisements