Issue 1, 2008

The dynamics and orientation of a lipophilic drug within model membranes determined by 13C solid-state NMR

Abstract

Methods for determining how a drug interacts with cellular membranes at the molecular level can give valuable insight into the mode of action of the drug and its absorption, distribution and metabolism profile. A procedure is described here to determine the orientation and location of the lipophilic drug trifluoperazine (TFP) intercalated into dimyristoylphosphatidylcholine (DMPC) bilayers, by using a novel combination of high-resolution solid-state nuclear magnetic resonance (SSNMR) methods to observe signals from 13C within the drug at natural abundance. SSNMR measurements of 1H–13C dipolar couplings for TFP and selective broadening of 13C NMR peaks by paramagnetic Mn2+ together suggest a model for the location, orientation and dynamics of the drug within lipid bilayers that offers an explanation for the lysoprotective effect of the drug at low concentrations. The experiments described are straightforward to implement and can be used for the routine analysis of drugmembrane interactions to provide useful information for drug design and structure refinement.

Graphical abstract: The dynamics and orientation of a lipophilic drug within model membranes determined by 13C solid-state NMR

Article information

Article type
Paper
Submitted
21 Aug 2007
Accepted
05 Oct 2007
First published
31 Oct 2007

Phys. Chem. Chem. Phys., 2008,10, 178-185

The dynamics and orientation of a lipophilic drug within model membranes determined by 13C solid-state NMR

M. P. Boland and D. A. Middleton, Phys. Chem. Chem. Phys., 2008, 10, 178 DOI: 10.1039/B712892D

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