Jump to main content
Jump to site search

Issue 9, 2008
Previous Article Next Article

Traction forces and rigidity sensing regulate cell functions

Author affiliations

Abstract

Increasing evidence suggests that mechanical cues inherent to the extracellular matrix may be as important as its chemical nature in regulating cell behavior. Here, the response of cells to the mechanical properties of the substrate is examined by culturing 3T3 fibroblastic cells and epithelial cells on surfaces composed of a dense array of flexible microfabricated pillars. We focus on the influence of substrate rigidity on the traction forces exerted by cells, and on cell adhesion and migration. We first measure these forces by monitoring the deflection of the pillars. Then, by varying their geometric parameters, we control the substrate stiffness over a large range from 1 to 200 nN μm−1. We show that the force–rigidity relationship exhibits a similar behavior for both cell types. Two distinct regimes are evidenced: first, a linear increase of the force with the rigidity and then a saturation plateau for the largest rigidities. We observe that the cell spreading area increases with increasing rigidity, as well as the size of focal adhesions. Substrates with an anisotropic rigidity allow us to determine that the migration paths of 3T3 cells are oriented in the stiffest direction in correlation with maximal traction forces. Finally, to compare the force measurements on micro-textured surfaces and continuous flexible gels, we propose an elastic model that estimates the equivalent Young's modulus of a micropillar substrate. This qualitative model gives comparable results for both experimental approaches.

Graphical abstract: Traction forces and rigidity sensing regulate cell functions

Back to tab navigation

Publication details

The article was received on 10 Mar 2008, accepted on 19 May 2008 and first published on 02 Jul 2008


Article type: Paper
DOI: 10.1039/B804103B
Soft Matter, 2008,4, 1836-1843

  •   Request permissions

    Traction forces and rigidity sensing regulate cell functions

    M. Ghibaudo, A. Saez, L. Trichet, A. Xayaphoummine, J. Browaeys, P. Silberzan, A. Buguin and B. Ladoux, Soft Matter, 2008, 4, 1836
    DOI: 10.1039/B804103B

Search articles by author

Spotlight

Advertisements