Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 22nd May 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 1, 2008
Previous Article Next Article

Protein, cell and bacterial fouling resistance of polypeptoid-modified surfaces: effect of side-chain chemistry

Author affiliations

Abstract

Peptidomimetic polymers consisting of poly-N-substituted glycine oligomers (polypeptoids) conjugated to biomimetic adhesive polypeptides were investigated as antifouling surface coatings. The polymers were immobilized onto TiO2 surfaces via an anchoring peptide consisting of alternating residues of 3,4-dihydroxyphenylalanine (DOPA) and lysine. Three polypeptoid side-chain compositions were investigated for antifouling performance and stability toward enzymatic degradation. Ellipsometry and XPS analysis confirmed that purified polymers adsorbed strongly to TiO2 surfaces, and the immobilized polymers were resistant to enzymatic degradation as demonstrated by mass spectrometry. All polypeptoid-modified surfaces exhibited significant reductions in adsorption of lysozyme, fibrinogen and serum proteins, and were resistant to 3T3 fibroblast cell attachment for up to seven days. Long-term in vitro cell attachment studies conducted for six weeks revealed the importance of polypeptoid side-chain composition, with a methoxyethyl side chain providing superior long-term fouling resistance compared to hydroxyethyl and hydroxypropyl side chains. Finally, attachment of both gram-positive and gram-negative bacteria for up to four days under continuous-flow conditions was significantly reduced on the polypeptoid-modified surfaces compared to unmodified TiO2 surfaces. The results reveal the influence of polypeptoid side-chain chemistry on short-term and long-term protein, cell and bacterial fouling resistance.

Graphical abstract: Protein, cell and bacterial fouling resistance of polypeptoid-modified surfaces: effect of side-chain chemistry

Back to tab navigation

Supplementary files

Publication details

The article was received on 03 Aug 2007, accepted on 19 Sep 2007 and first published on 12 Oct 2007


Article type: Paper
DOI: 10.1039/B711944E
Soft Matter, 2008,4, 131-139

  •   Request permissions

    Protein, cell and bacterial fouling resistance of polypeptoid-modified surfaces: effect of side-chain chemistry

    A. R. Statz, A. E. Barron and P. B. Messersmith, Soft Matter, 2008, 4, 131
    DOI: 10.1039/B711944E

Search articles by author

Spotlight

Advertisements