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Issue 5, 2008
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Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation

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Abstract

Techniques for stable, rapid and repeatable small-animal immobilization are necessary for high-throughput in vivo genetic/drug screens using cellular and sub-cellular features in multi-cellular organisms. We demonstrate a method for non-invasive and high-throughput on-chip immobilization of physiologically active C. elegans without the use of anesthesia or cooling, but with comparable stability even for the most demanding purposes. We show observation and manipulation of sub-cellular features in immobilized animals using two-photon microscopy and femtosecond-laser microsurgery.

Graphical abstract: Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation

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Submitted
20 Mar 2008
Accepted
28 Mar 2008
First published
02 Apr 2008

Lab Chip, 2008,8, 653-656
Article type
Communication

Sub-cellular precision on-chip small-animal immobilization, multi-photon imaging and femtosecond-laser manipulation

F. Zeng, C. B. Rohde and M. F. Yanik, Lab Chip, 2008, 8, 653
DOI: 10.1039/B804808H

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