Jump to main content
Jump to site search

Issue 6, 2008
Previous Article Next Article

Microfluidic device for label-free measurement of platelet activation

Author affiliations

Abstract

In this work we demonstrate a new microfluidic method for the rapid assessment of platelet size and morphology in whole blood. The device continuously fractionates particles according to size by displacing them perpendicularly to the fluid flow direction in a micro-fabricated post array. Whole blood, labeled with the fluorescent, platelet specific, antibody PE-anti-CD41, was run through the device and the positions of fluorescent objects noted as they exited the array. From this, histograms of platelet size were created which show marked increases in size after exposure to thrombin or a temperature of 4 °C. We infer that the well known morphological changes that occur during activation are causing the observed increase in size.

Graphical abstract: Microfluidic device for label-free measurement of platelet activation

Back to tab navigation

Article information


Submitted
15 Jan 2008
Accepted
19 Mar 2008
First published
11 Apr 2008

Lab Chip, 2008,8, 925-931
Article type
Paper

Microfluidic device for label-free measurement of platelet activation

D. W. Inglis, K. J. Morton, J. A. Davis, Thomas. J. Zieziulewicz, D. A. Lawrence, R. H. Austin and J. C. Sturm, Lab Chip, 2008, 8, 925
DOI: 10.1039/B800721G

Social activity

Search articles by author

Spotlight

Advertisements