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Issue 4, 2008
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A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities

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Abstract

This paper presents a new polymer lab-on-a-chip for magnetic bead-based immunoassay with fully on-chip sampling and detection capabilities, which provides a smart platform of magnetic immunoassay-based lab-on-a-chip for point-of-care testing (POCT) toward biochemical hazardous agent detection, food inspection or clinical diagnostics. In this new approach, the polymer lab-on-a-chip for magnetic bead-based immunoassay consists of a magnetic bead-based separator, an interdigitated array (IDA) micro electrode, and a microfluidic system, which are fully incorporated into a lab-on-a-chip on cyclic olefin copolymer (COC). Since the polymer lab-on-a-chip was realized using low cost, high throughput polymer microfabrication techniques such as micro injection molding and hot embossing method, a disposable polymer lab-on-a-chip for the magnetic bead-based immunoassay can be successfully realized in a disposable platform. With this newly developed polymer lab-on-a-chip, an enzyme-labelled electrochemical immunoassay (ECIA) was performed using magnetic beads as the mobile solid support, and the final enzyme product produced from the ECIA was measured using chronoamperometry. A sampling and detection of as low as 16.4 ng mL−1 of mouse IgG has been successfully performed in 35 min for the entire procedure.

Graphical abstract: A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities

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Supplementary files

Article information


Submitted
09 Oct 2007
Accepted
29 Jan 2008
First published
28 Feb 2008

Lab Chip, 2008,8, 542-549
Article type
Paper

A polymer lab-on-a-chip for magnetic immunoassay with on-chip sampling and detection capabilities

J. Do and C. H. Ahn, Lab Chip, 2008, 8, 542
DOI: 10.1039/B715569G

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