Issue 3, 2008
From the journal:

Journal of Analytical Atomic Spectrometry

ICP-MS-assisted proteomics approach to the identification of selenium-containing proteins in selenium-rich yeast†

Laure Tastet,a   Dirk Schaumlöffel*a  and  Ryszard Lobinskiab  

* Corresponding authors

a Laboratoire de Chimie Analytique Bio-Inorganique et Environnement, CNRS UMR 5254, Hélioparc, 2, av. Pr. Angot, F-64053 Pau, France
E-mail: dirk.schaumloeffel@univ-pau.fr
Fax: +33-559-407781
Tel: +33-559-407760

b Department of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664-Warsaw, Poland

Abstract

An ICP-MS-assisted proteomics approach was developed for the characterization of Se-containing proteins in selenium-rich yeast. Ultrasound probe leaching of a yeast sample with 8 M urea and 4% CHAPS and purification were optimized for highest selenium and protein extraction yield. The proteins were separated by 2D gel electrophoresis. The spots containing selenium (14) were located by laser ablation-ICP-MS, excised and analysed by nanoHPLC with the consecutive ICP-MS and electrospray Q-TOF MS/MS detection. The method allowed a successful identification of 17 selenium-containing proteins.

Graphical abstract: ICP-MS-assisted proteomics approach to the identification of selenium-containing proteins in selenium-rich yeast

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Article information

DOI
https://doi.org/10.1039/B713805A
Article type
Paper
Submitted
07 Sep 2007
Accepted
30 Oct 2007
First published
20 Nov 2007

J. Anal. At. Spectrom., 2008,23, 309-317

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ICP-MS-assisted proteomics approach to the identification of selenium-containing proteins in selenium-rich yeast

L. Tastet, D. Schaumlöffel and R. Lobinski, J. Anal. At. Spectrom., 2008, 23, 309 DOI: 10.1039/B713805A

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