Jump to main content
Jump to site search

Issue 3, 2008
Previous Article Next Article

ICP-MS-assisted proteomics approach to the identification of selenium-containing proteins in selenium-rich yeast

Author affiliations

Abstract

An ICP-MS-assisted proteomics approach was developed for the characterization of Se-containing proteins in selenium-rich yeast. Ultrasound probe leaching of a yeast sample with 8 M urea and 4% CHAPS and purification were optimized for highest selenium and protein extraction yield. The proteins were separated by 2D gel electrophoresis. The spots containing selenium (14) were located by laser ablation-ICP-MS, excised and analysed by nanoHPLC with the consecutive ICP-MS and electrospray Q-TOF MS/MS detection. The method allowed a successful identification of 17 selenium-containing proteins.

Graphical abstract: ICP-MS-assisted proteomics approach to the identification of selenium-containing proteins in selenium-rich yeast

Back to tab navigation

Supplementary files

Publication details

The article was received on 07 Sep 2007, accepted on 30 Oct 2007 and first published on 20 Nov 2007


Article type: Paper
DOI: 10.1039/B713805A
J. Anal. At. Spectrom., 2008,23, 309-317

  •   Request permissions

    ICP-MS-assisted proteomics approach to the identification of selenium-containing proteins in selenium-rich yeast

    L. Tastet, D. Schaumlöffel and R. Lobinski, J. Anal. At. Spectrom., 2008, 23, 309
    DOI: 10.1039/B713805A

Search articles by author

Spotlight

Advertisements