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Issue 6, 2008
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DNAzymes for sensing, nanobiotechnology and logic gate applications

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Abstract

Catalytic nucleic acids (DNAzymes or ribozymes) are selected by the systematic evolution of ligands by exponential enrichment process (SELEX). The catalytic functions of DNAzymes or ribozymes allow their use as amplifying labels for the development of optical or electronic sensors. The use of catalytic nucleic acids for amplified biosensing was accomplished by designing aptamer–DNAzyme conjugates that combine recognition units and amplifying readout units as in integrated biosensing materials. Alternatively, “DNA machines” that activate enzyme cascades and yield DNAzymes were tailored, and the systems led to the ultrasensitive detection of DNA. DNAzymes are also used as active components for constructing nanostructures such as aggregated nanoparticles and for the activation of logic gate operations that perform computing.

Graphical abstract: DNAzymes for sensing, nanobiotechnology and logic gate applications

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Publication details

The article was received on 20 Feb 2008 and first published on 24 Apr 2008


Article type: Tutorial Review
DOI: 10.1039/B718428J
Chem. Soc. Rev., 2008,37, 1153-1165

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    DNAzymes for sensing, nanobiotechnology and logic gate applications

    I. Willner, B. Shlyahovsky, M. Zayats and B. Willner, Chem. Soc. Rev., 2008, 37, 1153
    DOI: 10.1039/B718428J

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