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Issue 46, 2008
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Gold single-crystal electrode surface modified with self-assembled monolayers for electron tunneling with bilirubin oxidase

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Abstract

Using Au(111) and Au(100) single-crystal electrodes modified with self-assembled monolayers (SAMs), the direct electron transfer reaction of bilirubin oxidase (BOD) adsorbed onto their surfaces was investigated. The BOD adsorbed onto the Au(111), Au(100) and gold /mica electrodes, and the BOD adsorbed onto Au(111) electrodes modified with C3–SO3H and CnCOOH (n = 2, 5 and 7), showed the electrocatalytic currents of dioxygen reduction based on the direct electron transfer reaction. The BOD adsorbed onto Au(111) electrodes modified with C6–NH2, C6OH and C5–CH3 did not show any electrocatalytic current. Negatively charged electrode surfaces can give a suitable molecular orientation for the direct electron transfer of BOD. The k° values evaluated by an analysis of the steady-state voltammogram with a simulated fitting method did not depend on the crystal structure of the gold electrode surface. Using a CnCOOH (n = 2, 5, 7) modified Au(111) electrode, the k° values decreased with an increasing alkyl chain length of CnCOOH. Based on the k° values obtained from the CnCOOH (n = 2, 5, 7) modified Au(111) electrodes, the electron tunneling distance was evaluated. The average distance between the type 1 Cu site of BOD and the outside of the BOD protein structure was evaluated to be 17 (±2) Å.

Graphical abstract: Gold single-crystal electrode surface modified with self-assembled monolayers for electron tunneling with bilirubin oxidase

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Article information


Submitted
09 Jun 2008
Accepted
26 Aug 2008
First published
14 Oct 2008

Phys. Chem. Chem. Phys., 2008,10, 6928-6934
Article type
Paper

Gold single-crystal electrode surface modified with self-assembled monolayers for electron tunneling with bilirubin oxidase

M. Tominaga, M. Ohtani and I. Taniguchi, Phys. Chem. Chem. Phys., 2008, 10, 6928
DOI: 10.1039/B809737B

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